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Elimination is achieved by means of a mobile phase, typically combination of water and organic solvents (such as acetonitrile or methanol) are sprayed onto the column in the form of gradient, such as 95:5 aqueous: organic, for example and gradually increasing the organic phase until the elution buffer is 5:95 organic: aqueous)

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After intense workouts, muscles undergo tissue breakdown and need to be repaired for growth