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(2)制备Fmoc-Leu-Val-WangResin (2) Preparation of Fmoc-Leu-Val-WangResin 在步骤(1)的Fmoe-Val-WangResin中，加入脱帽试剂(20％哌啶的DMF溶液)，常温反应5分钟，抽干，重新加入脱帽剂常温反应20分钟，用异丙醇洗涤3次，DMF洗涤3次，重蒸过的DMF洗涤2次，抽干，加入Fmoc-Leu-OH 0.7g，DIEA0.4ml，TBTU0.56g，HOBT0.2g，DMF10ml常温反应1.5小时，用真空泵抽干，用异丙醇洗涤3次，DMF洗涤3次，重蒸过的DMF洗涤2次，抽干，获得Fmoc-Leu-Val-WangResin； In the Fmoe-Val-WangResin of step (1), add decapping reagent (DMF solution of 20% piperidine), react at room temperature for 5 minutes, drain, add decapping agent at room temperature and react for 20 minutes, wash 3 times with isopropanol , washed 3 times with DMF, washed 2 times with re-distilled DMF, drained, added 0.7g of Fmoc-Leu-OH, 0.4ml of DIEA, 0.56g of TBTU, 0.2g of HOBT, 10ml of DMF, reacted at room temperature for 1.5 hours, and dried with a vacuum pump