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Primary antibodies targeting specific proteins were employed at the following dilutions: CPT1A (1:1,000, Proteintech, #66039-1-Ig), NLRP3 (1:1,000, BOSTER, #BA3677), cleaved Caspase-1 (1:1,000, Cell Signaling Technology, #89332), GSDMD (1:1,000, Affinity, AF4012), β-Actin (1:10,000, ABclonal, #AC038), goat anti-rabbit (1:10,000, Proteintech, #SA00001-2) and goat anti-mouse (1:10,000, Proteintech, #SA00001-1)

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However, under OS conditions, ROS binds to KEAP1, inducing its conformational change that leads to Nrf2 nuclear translocation and increased expression of antioxidant enzymes

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