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11, 29 Although it is unclear whether the accumulation of iron in the brain is primary or secondary to the development of neurodegenerative disorders, 30 one animal study has shown that unilateral injection of FeCl 3 into the substantia nigra of adult rats results in a 95% decrease of striatal dopamine, which impaired dopamine-related behavioral responses

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For the differentiation of Th1 cells, the isolated naive CD4 + T cells were cultured in RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum (Serana, #S-FBS-SA-015, Brandenburg, Germany), 100 U/ml penicillin & 100 µg/ml streptomycin (Thermo Fisher Scientific, #15070063), 10 mM HEPES (Thermo Fisher Scientific, #15630106), 0.05 mM 2-mercaptoethanol (Thermo Fisher Scientific, #21985023), 10 ng/mL IL-12 (Peprotech, #577002), 5 μg/mL anti-IL-4 (Biolegend, #504122)