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PhoA accumulation was analyzed by SDS-PAGE (NuPAGE™ 4 bis 12%, Bis-Tris, Invitrogen, Waltham, MA, USA) and Western blot analyses were carried out using nitrocellulose membranes in the iBLOT2 system (Invitrogen, Waltham, MA, USA), both according to the supplier
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