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Cells were used within three months post-thawing and cultured in RPMI 1640 medium (#01859-34, Nacalai Tesque, Kyoto, Japan) with 10% fetal bovine serum (#30264-85, Nacalai Tesque, Kyoto, Japan), 2 mM glutamine, 50 U/mL penicillin, and 100 µg/mL streptomycin (#26253-84, Nacalai Tesque, Kyoto, Japan)
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Alterations in Ca 2+ handling via the MCU have been observed in multiple recent studies performed in iPSC-derived DA neurons from PD patients harboring mutations in LRRK2, GBA1, Parkin SNCA and GBA1 as evidenced by an increase in cytosolic Ca 2+ [213, 214]
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