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Lastly, GHR signaling has also been associated with activation of the phosphatidylinositol-3 kinase (PI3K)/Akt pathway in a Jak2/IRS-1 dependent manner (Liang et al., 2000)

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Variant:WA10-SINGLE

The internal standard (4-methylpentanoic acid) was prepared into 375 μg/mL with ether, Ten standard curve points were prepared by mixing 200 μL working standard solution of series of 6 acids, 100 μL 15% phosphoric acid, 20 μL working standard solution of series of hexanoic acid, 20 μL internal standard and 260 μL ethyl ether at the following concentrations: 0.02, 0.1, 0.5, 2, 10, 25, 50, 100, 250, and 500 μg/mL 100 μL of 15% phosphoric acid, 20 μL of hexanoic acid working standard solution, 20 μL of the internal standard, and 260 μL of ether