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There are a variety of approaches to induce angiogenesis in engineered tissue: strategies such as scaffold functionalization (including engineered tissue with angiogenic factors), cell-based technique (the addition of pro-angiogenic expressing cells to artificial tissues), bioreactors (containing rotating and perfusion bioreactors that are responsible for transferring angiogenic factors and other nutrients to artificial tissue), microfluidic systems (artificial design of vascular networks), modular assembly (an evolved form of cell-based techniques with the difference that a layer of endothelial cells is embedded around a prefabricated polymer, and eventually the system is added to the engineered tissue as artificial vessels), and the latest strategy, in vivo systems

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Reduction of colitis by prebiotics in HLA-B27 transgenic rats is associated with microflora changes and immunomodulation